Laboratory Testing Directory
Laboratoy Testing Directory
Blood Sample Requirements: Blood Samples are accepted from 7:00 AM Monday till 2:00 PM Friday.
Federal regulations require all clinical blood specimens (tubes, containers) be labeled with the patient's full name (spelled correctly), birth date and sample date/time. If these requirements are not met, the lab must delay or refuse testing until compliance is met.
For pediatric sample volume contact the Clinical Laboratory.
A specimen may be rejected for any of the following reasons:
• Incorrect or compromised specimen (hemolysis, clotted specimens)
• Insufficient volume for analysis
• Unlabeled or improperly labeled specimen
• Specimen that has been sent in incorrect collection tube(s)
• Incomplete or incorrect testing requisition
Immunogenetics Testing
Description: Determination of ABO blood group
Methodology: Serology
Test Code(s): ABO
Days Performed: Monday - Friday, 0630-1630
Turn Around Time: 1 Day
STAT Turn Around Time: 1 Hour
Collection Requirements
Specimen: Whole Blood
Collection Container: (1) Lavender Top (EDTA). Also Acceptable: (1) Yellow Top (ACD-A)
Collection Volume: 6 mL
Specimen Processing Requirements
Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Test Limitations: Grossly hemolyzed samples will be rejected.
Description: Test is used for the detection and identification of antibodies directed to donor-specific HLA class I and II antigens by single-antigen bead-based flow cytometry.
Methodology: Luminex Microbead Array
Test Code(s): PTXSAB1Fee, PTXSAB2Fee
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
STAT Turn Around Time: 24 Hours
Collection Requirements
Specimen: Whole Blood
Collection Container: (1) Red Top
Collection Volume: 6-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
The laboratory will report mean fluorescence intensity (MFI) of beads bearing HLA antigen specific to the broad antigens mismatched to the transplant organ donor. Information may be useful in tracking and trending sensitization (or desensitization) to donor HLA antigens.
Test Limitations: Donor HLA typing required for interpretation.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: Detection of the presence of autoantibodies (HLA) in a solid organ recipient.
Methodology: Flow Cytometry
Test Code(s): X-Auto T-Cell, X-Auto B-Cell
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
Collection Requirements
Specimen: Blood
Collection Container: (3) ACD Tubes (Yellow Top), (1) Red Top
Collection Volume: 5-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
The laboratory will report crossmatch results "positive," "weak positive," or "negative" based on the change in fluorescent signal (median channel shift (MCS)). Any WEAK POSITIVE or POSITIVE result is directly approved by the Medical Director before reporting.
Test Limitations: A proper interpretation of crossmatch testing requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and/or interfering medications, substances (i.e., Rituximab Therapy) is provided to the laboratory PRIOR to testing any serum.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: Evaluate the compatibility between potential transplant recipient and living donor.
Methodology: Flow Cytometry
Test Code(s): X-LD T-Cell, X-LD B-Cell
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
STAT Turn Around Time: 4-6 Hours
Collection Requirements
Specimen: Blood
Collection Container: Donor - (3) ACD Tubes (Yellow Top), Patient - (1) Red Top
Collection Volume: 6-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
The laboratory will report crossmatch results “positive,” “weak positive,” or “negative,” based on the change in fluorescent signal (median channel shift (MCS)). Results should be interpretated in conjunction with Luminex antibody testing and clinical risk factors. Positive flow crossmatch may indicate increased risk of antibody mediated organ rejection. Any WEAK POSITIVE or POSITIVE result is directly approved by the Medical Director before reporting.
Test Limitations: A proper interpretation of crossmatch testing requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., Rituximab Therapy) is provided to the laboratory PRIOR to testing any serum.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: Single Antigen Antibody specificities to HLA Class I antigens.
Methodology: Luminex Microbead Array
Test Code(s): SAB1
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
STAT Turn Around Time: 6 Hours
Collection Requirements
Specimen: Whole Blood
Collection Container: (1) Red Top
Collection Volume: 5-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
Results will list class I (HLA-A, -B, or -C locus) antibodies detected and the calculated percent reactive antibody (cPRA).
Test Limitations: A proper interpretation of antibodies requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., IVIG) is provided to the laboratory PRIOR to testing any serum.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: Single Antigen Antibody specificities to HLA Class II antigens.
Methodology: Luminex Microbead Array
Test Code(s): SAB2
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
STAT Turn Around Time: 6 Hours
Collection Requirements
Specimen: Whole Blood
Collection Container: (1) Red Top
Collection Volume: 5-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
Results will list class II (HLA-DR, -DQ, or -DP locus) antibodies detected and the calculated percent reactive antibody (cPRA).
Test Limitations: A proper interpretation of antibodies requires that all potential sensitization events (i.e., Transfusions, Pregnancies, etc.) and / or interfering medications, substances (i.e., IVIG) is provided to the laboratory PRIOR to testing any serum.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: DNA based assay for HLA-A, B, C, DR, DR345 and DQB (Low resolution).
Methodology: Reverse SSO detected by Luminex xMAP
Test Code(s): T-HLA CL1+2
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 3-7 Days
STAT Turn Around Time: 6 Hours
Collection Requirements
Specimen: Whole Blood
Collection Container: (2) Yellow Top (ACD-A), Also Acceptable: (2) Lavender Top (EDTA)
Collection Volume: 5-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
An HLA typing report will be provided.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: DNA based assay for HLA-DQA, DPA, DPB (Low Resolution)
Methodology: Reverse SSO detected by Luminex xMAP
Test Code(s): T-HLA CL2 Ext
Days Performed: Monday-Friday, 0630-1630
Turn Around Time: 2-5 Days
STAT Turn Around Time: 6 Hours
Collection Requirements
Specimen: Whole Blood
Collection Container: (2) Yellow Top (ACD-A), Also Acceptable: (2) Lavender Top (EDTA)
Collection Volume: 5-10 mL
Specimen Processing Requirements
Specimen Transport: Specimen Transport: Transport specimen(s) at room temperature. Ship according to Department of Transportation regulations for biological specimens. Ship samples "Next Day" delivery or via courier.
Interpretation
An HLA typing report will be provided.
Additional Information: Any questions or results can be addressed to the HLA lab and/or Medical Director.
This test was developed, and its performance characteristics determined by Nevada Donor Network Histocompatibility Laboratory. It has not been cleared or approved by the US Food and Drug Administration (FDA).
Description: Reduced reporting time, MUST contact the laboratory to schedule
Test Code(s): Z-STAT Fee
Days Performed: Monday-Friday, 0630-1630
STAT Turn Around Time: 6 Hours
Description: Reduced reporting time, MUST contact the laboratory to schedule
Test Code(s): Z-After Hours
Days Performed: Monday-Friday after 1630, includes weekends
STAT Turn Around Time: 6 Hours
Surgical Pathology
Opening Fall 2023